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rat bbb culture kit  (PharmaCo Cell Company Ltd)
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PharmaCo Cell Company Ltd rat bbb culture kit
(A) Schematic representation of an in vitro <t>BBB</t> model constructed from primary cultures of rat brain capillary endothelial cells, brain pericytes, and astrocytes. Synthetic <t>plant-type</t> <t>ceramides</t> (5 μM) were added to the medium in the inserts. After 30 min, conditioned medium in the bottom wells was collected and analyzed by LC-MS/MS. (B) Chemical structure of d18:2/C5-ceramide. (B) Chemical structures of d18:2/C6- and d18:2/C15-ceramides. (C) Levels of d18:2/C6-ceramide and d18:2/C15-ceramide. Data are presented as means ± SD (n = 3; * p <0.05 by t-test. (D) Levels of d18:2/C6-SM, d18:2/C15-SM, and GlcCer. Data are represented as means ± SD (n = 3; ns, not significant; * p <0.05 by t-test).
Rat Bbb Culture Kit, supplied by PharmaCo Cell Company Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rat+bbb+culture+kit/pmc07605672-47-11-16?v=PharmaCo+Cell+Company+Ltd
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rat bbb culture kit - by Bioz Stars, 2026-08
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(A) Schematic representation of an in vitro BBB model constructed from primary cultures of rat brain capillary endothelial cells, brain pericytes, and astrocytes. Synthetic plant-type ceramides (5 μM) were added to the medium in the inserts. After 30 min, conditioned medium in the bottom wells was collected and analyzed by LC-MS/MS. (B) Chemical structure of d18:2/C5-ceramide. (B) Chemical structures of d18:2/C6- and d18:2/C15-ceramides. (C) Levels of d18:2/C6-ceramide and d18:2/C15-ceramide. Data are presented as means ± SD (n = 3; * p <0.05 by t-test. (D) Levels of d18:2/C6-SM, d18:2/C15-SM, and GlcCer. Data are represented as means ± SD (n = 3; ns, not significant; * p <0.05 by t-test).

Journal: PLoS ONE

Article Title: Blood-brain barrier permeability analysis of plant ceramides

doi: 10.1371/journal.pone.0241640

Figure Lengend Snippet: (A) Schematic representation of an in vitro BBB model constructed from primary cultures of rat brain capillary endothelial cells, brain pericytes, and astrocytes. Synthetic plant-type ceramides (5 μM) were added to the medium in the inserts. After 30 min, conditioned medium in the bottom wells was collected and analyzed by LC-MS/MS. (B) Chemical structure of d18:2/C5-ceramide. (B) Chemical structures of d18:2/C6- and d18:2/C15-ceramides. (C) Levels of d18:2/C6-ceramide and d18:2/C15-ceramide. Data are presented as means ± SD (n = 3; * p <0.05 by t-test. (D) Levels of d18:2/C6-SM, d18:2/C15-SM, and GlcCer. Data are represented as means ± SD (n = 3; ns, not significant; * p <0.05 by t-test).

Article Snippet: To evaluate the in vitro BBB permeability of plant ceramides, a Rat BBB Culture Model Kit (PharmaCo-Cell Co. Ltd., Nagasaki, Japan), which consist of primary cultures of brain capillary endothelial cells, brain pericytes and astrocytes [ , ], was used.

Techniques: In Vitro, Construct, Liquid Chromatography with Mass Spectroscopy